Acyl-group specificity of AHL synthases involved in quorum-sensing in Roseobacter group bacteria

• Lisa Ziesche,
• Jan Rinkel,
• Jeroen S. Dickschat and
• Stefan Schulz

Beilstein J. Org. Chem. 2018, 14, 1309–1316, doi:10.3762/bjoc.14.112

• ; fatty acid composition; N-acylhomoserine lactones; quorum sensing; Phaeobacter inhibens; Introduction The Roseobacter group, a subgroup of the Rhodobacteraceae family, constitutes an important class of Gram-negative marine bacteria, occurring in many different habitats [1][2], in fresh water as well as

• available? In the latter case the presence of the acyl precursors would determine the structure of the final AHL. To answer this question, the fatty acid composition of the native roseobacters was determined and compared to the AHLs produced. In addition, LuxI-type enzymes were heterologously expressed in E

• unsaturation in the alkyl chain towards the carboxy terminus. These data indicate this FAME to be 2,11-C18:1, the parent acid of the major D. shibae AHL, 2E,11Z-C18:2-HSL [10]. The analysis performed with bacteria grown in liquid medium led to comparable results, indicating that the fatty acid composition does

Effects of RAMEA-complexed polyunsaturated fatty acids on the response of human dendritic cells to inflammatory signals

• Éva Rajnavölgyi,
• Renáta Laczik,
• Viktor Kun,
• Lajos Szente and
• Éva Fenyvesi

Beilstein J. Org. Chem. 2014, 10, 3152–3160, doi:10.3762/bjoc.10.332

• diseases [7]. Intake of dietary fats may influence inflammation in the gastrointestinal tract. In a long-term study conducted on 170 805 women a reduced risk of ulcerative colitis was observed for the participants with high intake of n−3 PUFAs [8]. The fatty acid composition of the diet, in particular, the

• proportion of different types of PUFAs, has an influence on the fatty acid composition of immune cells which is a chemical trigger for immune response, such as inflammation [9]. Supplementation of the diet of healthy human volunteers with n−3 PUFAs was unambiguously beneficial: the number of monocytes and